Microplate Readers and Reader Accessories
Microplate readers are a type of spectrophotometer designed specifically for analyzing multiple samples in multi-well plates.
The readers detect light signals that result from biological, chemical, biochemical, or physical reactions. The signals are produced by, converted by, or transmitted through the samples and are measured by a detector. The detectors are usually photomultiplier tubes (PMT), which convert the released photons into electricity. The current is quantified by the reader’s software to produce the numerical result.
In some cases, samples are first excited by exposure to light of specific wavelengths. Filters or monochromators are used to select or restrict the wavelengths needed for emission or detection.
Single-mode microplate readers commonly measure absorbance, fluorescence, or luminescence. Multi-mode plate readers may measure absorbance, fluorescence, and luminescence with options for fluorescence polarization (FP). They may also include lasers for time-resolved fluorescence (TRF), TR-FRET, Amplified Luminescent Proximity Homogeneous Assays (ALPHA), and cell imaging.
Features may include:
- Monochromator- or filter-based wavelength selection
- Visible only or UV/Vis spectrum, fluorescence, and luminescence
- Multiple photomultiplier tubes (PMTs) for simultaneous assays
- Temperature and CO2/O2controls
- Modular design for adding functionality
Microplate readers are used for high-throughput screening (HTS), biomarker assays, nucleic acid and protein quantification, kinetics, ELISA assays, homogeneous time resolved fluorescence (HTRF) for measuring cytokines, bioluminescence resonance energy transfer (BRET), and other techniques.
Revvity Health Sciences Inc HTRF Human Total PINK1 Detection Kit, 500 Assay Points
HTRF Human Total PINK1 Detection Kit, 500 Assay Points
Revvity Health Sciences Inc HTRF Human Phospho-Cyclin D1 (Thr286) Detection Kit, Control Lysate
HTRF Human Phospho-Cyclin D1 (Thr286) Detection Kit, Control Lysate
Revvity Health Sciences Inc HTRF Human & Mouse Fibronectin Detection Kit, 500 Assay Points
HTRF Human & Mouse Fibronectin Detection Kit, 500 Assay Points
Sigma Aldrich Fine Chemicals Biosciences PyrexR low actinic media s1L
Heavy-duty low actinic amber glass bottles protect light-sensitive materials. Include linerless one-piece autoclavable orange PP plug-seal caps (size GL 45) with drip-free pouring rings. Permanent white enamel graduations and marking spots.
Molecular Devices Polarization Emission Filter, Visible, Pair, For FilterMax Readers
Replacement polarization emission filter set for fluorescence polarization measurements in multi-mode microplate readers. Supplied as a pair (quantity 2), these emission filters are intended to enable polarization-based fluorescence detection and improve signal discrimination in applicable assays.
- Replacement emission filters for fluorescence polarization assays.
- Visible emission band, vendor designation "Visible".
- Sold as a pair (quantity 2) for filter-based reader configurations.
- Intended for compatible multi-mode microplate readers.
- Helps improve polarization signal discrimination and assay reproducibility.
Molecular Devices Polarization Filter, 700 nm BW 60 nm, Vertical
This fluorescence polarization filter in a holder enables near-infrared fluorescence polarization measurements centered at 700 nm and installs into the filter slot of compatible multi-mode microplate readers to support FP assays.
- 700 nm center wavelength with 60 nm bandwidth.
- Fluorescence polarization optical design for polarization-based measurements.
- Vertical polarization orientation for polarization detection.
- Mounted in a holder for insertion into reader filter slots.
- Intended for fluorescence polarization assays and near-IR detection workflows.
Revvity Health Sciences Inc HTRF Human Total PR Detection Kit, 10,000 Assay Points
HTRF Human Total PR Detection Kit, 10,000 Assay Points
Revvity Health Sciences Inc HTRF Human Androgen Receptor Variant 7 Detection Kit, 500 Assay Points
HTRF Human Androgen Receptor Variant 7 Detection Kit, 500 Assay Points
Revvity Health Sciences Inc AlphaLISA SureFire Ultra Human Phospho-c-FOS (Ser32) Detection Kit, 100 Assay Points
AlphaLISA SureFire Ultra Human Phospho-c-FOS (Ser32) Detection Kit, 100 Assay Points
Revvity Health Sciences Inc HTRF Human Total JAK1 Detection Kit, 10,000 Assay Points
HTRF Human Total JAK1 Detection Kit, 10,000 Assay Points
Revvity Health Sciences Inc HTRF Human Phospho-c-RAF (Ser43) Detection Kit, 50,000 Assay Points
HTRF Human Phospho-c-RAF (Ser43) Detection Kit, 50,000 Assay Points
Molecular Devices Excitation Filter, 535 nm, 25 nm Bandwidth
This excitation filter provides a 535 nm center wavelength with a 25 nm full-width bandwidth for fluorescence excitation in microplate assays. It is intended for use with multi-mode fluorescence microplate readers to enable selective excitation of fluorophores around 535 nm.
- Center wavelength 535 nm.
- Bandwidth 25 nm full width.
- Excitation filter for fluorescence detection.
- Compatible with multi-mode microplate and ELISA readers.
- Designed for microplate-based fluorescence assays.