Microplate Readers and Reader Accessories
Microplate readers are a type of spectrophotometer designed specifically for analyzing multiple samples in multi-well plates.
The readers detect light signals that result from biological, chemical, biochemical, or physical reactions. The signals are produced by, converted by, or transmitted through the samples and are measured by a detector. The detectors are usually photomultiplier tubes (PMT), which convert the released photons into electricity. The current is quantified by the reader’s software to produce the numerical result.
In some cases, samples are first excited by exposure to light of specific wavelengths. Filters or monochromators are used to select or restrict the wavelengths needed for emission or detection.
Single-mode microplate readers commonly measure absorbance, fluorescence, or luminescence. Multi-mode plate readers may measure absorbance, fluorescence, and luminescence with options for fluorescence polarization (FP). They may also include lasers for time-resolved fluorescence (TRF), TR-FRET, Amplified Luminescent Proximity Homogeneous Assays (ALPHA), and cell imaging.
Features may include:
- Monochromator- or filter-based wavelength selection
- Visible only or UV/Vis spectrum, fluorescence, and luminescence
- Multiple photomultiplier tubes (PMTs) for simultaneous assays
- Temperature and CO2/O2controls
- Modular design for adding functionality
Microplate readers are used for high-throughput screening (HTS), biomarker assays, nucleic acid and protein quantification, kinetics, ELISA assays, homogeneous time resolved fluorescence (HTRF) for measuring cytokines, bioluminescence resonance energy transfer (BRET), and other techniques.
Revvity Health Sciences Inc HTRF Human Phospho-ATM (Ser1981) Detection Kit, 10,000 Assay Points
HTRF Human Phospho-ATM (Ser1981) Detection Kit, 10,000 Assay Points
Molecular Devices Replacement Lamp Pack, Microplate Reader, 2-Lamp, 5 V
Replacement lamp pack for use with Molecular Devices Emax and Vmax microplate readers. Contains two 5 V lamps to restore the reader's illumination for optical assays.
- Restores instrument light output for absorbance and fluorescence measurements.
- Includes two replacement lamps per package for complete lamp replacement.
- 5 V lamp specification designed for microplate reader lamp assemblies.
- Field-replaceable bulbs simplify routine maintenance.
Molecular Devices Fluorescence Polarization Filter, 485 nm, 20 nm, Horizontal
Fluorescence polarization filter with holder designed for fluorescence polarization assays. The filter provides excitation at 485 nm with a 20 nm bandwidth and a horizontal polarized orientation. It installs in standard filter slides used by multi-mode microplate readers.
- Excitation center wavelength 485 nm with 20 nm bandwidth.
- Polarized horizontal orientation for fluorescence polarization assays.
- Includes filter holder compatible with standard filter slides.
- Intended for use with multi-mode microplate readers in fluorescence polarization workflows.
- Suitable for assays requiring excitation near 485 nm.
Revvity Health Sciences Inc AlphaLISA AAV3B Detection Kit, 5,000 Assay Points
AlphaLISA AAV3B Detection Kit, 5,000 Assay Points
Revvity Health Sciences Inc HTRF Human Total ATF4 Detection Kit, 10,000 Assay Points
HTRF Human Total ATF4 Detection Kit, 10,000 Assay Points
Revvity Health Sciences Inc AlphaLISA SureFire Ultra Human Total TIE2 Detection Kit, 100 Assay Points
AlphaLISA SureFire Ultra Human Total TIE2 Detection Kit, 100 Assay Points
Molecular Devices Absorbance Filter, 450 nm, 8 nm, FilterMax Compatible
Replaceable narrow-band absorbance (photometric) filter designed for use in FilterMax multimode microplate readers. The filter is centered at 450 nm and has an approximate 8 nm bandwidth, providing a specific narrow bandpass for fixed-wavelength absorbance measurements in microplates.
- Center wavelength: 450 nm.
- Bandwidth: 8 nm (approximate).
- Designed for fixed-wavelength absorbance assays.
- Installs in the instrument filter wheel for modular wavelength selection.
- Narrow-bandpass optical filter for consistent photometric readings.
Molecular Devices Excitation Filter, 360 nm, 35 nm Bandwidth
The Molecular Devices 360 nm fluorescence excitation filter is an accessory for FilterMax series multi-mode microplate readers, providing a narrow excitation band centered at 360 nm for assays that require near-UV excitation.
- Provides 360 nm excitation with approximately 35 nm bandwidth.
- Designed for fluorescence assays on microplate readers and ELISA readers.
- Fits FilterMax series reader filter holders for standard microplate formats.
- Improves signal-to-noise for near-UV-excited fluorophores.
- Simple to install and replace during routine maintenance.
Electron Microscopy Sciences NIGHTSEA™ Fluorescence Adapter VI Light Head, Ultra Violet
Additional combinations excitation/ emission wavelength combination can be added NIGHTSEA™ Stereo Microscope Fluorescence Viewing system by purchasing individual light head, barrier filter, and viewing shield combinations. These three elements can be removed and replaced in seconds, and color coding ensures that you are using the right combination. The barrier filter clicks on to the ring adapter magnetically, so it is easy to remove it to switch back to white light viewing.
Color: Ultra Violet
Excitation: 360-380 nm
Emission filter: 415 nm longpass
Fluorophores: DAPI
Revvity Health Sciences Inc HTRF FcRn Binding Kit, 10,000 Assay Points
HTRF FcRn Binding Kit, 10,000 Assay Points